Core Principles and Clinical Applications of EBOO Regenerative Medicine Therapy

Extracorporeal Blood Oxygenation and Ozonation (EBOO) is the modern, minimally invasive treatment developed through combining modern techniques of extracorporeal blood circulation, hyperbaric oxygenation, and controlled medical ozone therapy. It represents the advanced modified version of the traditional autohemotherapy which allows performing the complete oxygenation and standardized ozone modulation of the whole amount of blood in the human organism, thus allowing to achieve systemic metabolic regulation, immunomodulation, and detoxification effects. It has gained wide recognition in the field of complementary and regenerative medicine and has become a hot topic in searching for non-pharma treatment solutions for medical specialists and patients around the globe.

1. Key Definitions of EBOO
   EBOO is the process where the venous blood of the patients is taken out into a closed extracorporeal circuit, the blood is fully oxygenated with the help of high-purity medical oxygen, and the blood is exposed to the special low-concentration ozone-oxygen mixture. The blood is returned into the patient only after the blood has been completely processed and it underwent gas exchange together with purification from impurities.
   Unlike the ordinary minor ozone autohemotherapy allowing for the treatment of a limited blood volume (100–200 mL) only, EBOO allows blood processing in a continuous way providing treatment for the blood volume in one session. The standard equipment used for EBOO is represented by the closed sterile pipeline system which eliminates cross-infection and guarantees the safety of the treatment.
2. The Principle of EBOO Work
   The therapeutic effect achieved with the help of the method of EBOO is based on the synergistic effect of the action of oxygen and gentle ozone oxidation, which triggers a positive response in the human body without causing any oxidative damage.The fundamental processes of EBOO can be outlined in three sections:
   2.1 Blood Purification and Oxygenation
   Extracorporeal hyperoxygenation achieves rapid raise in the partial pressure of oxygen in blood and saturation of oxygen in hemoglobin, enhancing the microcirculation and alleviating oxygen starvation of tissues. The closed circuit is able to remove metabolic waste, inflammatory messengers, and lipid deposits from the blood, thereby decreasing viscosity and increasing fluidity.
   2.2 Mild Oxidative Eustress and Activation of Antioxidants
   The medical ozone in exact dosages acts as a mild oxidative stress factor and connects with polyunsaturated fatty acids in the plasma activating antioxidant responsive element (ARE) in human DNA. This chain of events results in the expression of about 200 detoxifying and antioxidant genes increasing the activity of the main antioxidants including superoxide dismutase, glutathione reductase, and catalase. It helps the human organism improve the endogenous antioxidant potential and stop chronic oxidative stress effects.
   2.3 Immune and Metabolic Modulation
   EBOO is able to control the production of different immune cells making inflammatory processes less intense and influencing the removal of apoptotic cells and other by-products. Additionally, the procedure improves lipid metabolism, decreases lipid deposits in blood, and enhances the work of endothelial cells making ways to fight chronic vascular diseases.
3. Standard EBOO Procedure
   EBOO is a standardized clinical treatment carried out under the supervision of qualified specialists following established sterile protocols. The entire procedure is safe, non-invasive, and takes approximately 40 to 60 minutes.1. Uninterrupted Circulation of Blood: A precise peristaltic pump causes continuous flow of blood through the pump preventing blood cell damage.
4. Oxygen-Ozone Treatment: The blood comes in contact with the oxygen-ozone prepared mixture through countercurrent gas exchange in the reactor to get fully oxidized and oxygenated.
5. Filtration and Purification: The circulating blood passes the precision filter for the removal of large molecular waste, microthrombi, and inflammation-causing substances.
6. Sterile Reinfusion: The processed and oxygen-rich blood is reinfused into the patient’s body completing a treatment cycle.
7. Usage of EBOO
   The efficacy of EBOO has been clinically confirmed in many clinical trials and laboratory experiments of animals and is used in many branches of medicine all over the world:

• Vascular diseases: Severe ischemia, disorders of coronary microcirculation, cholesterol embolism, and syndrome of high viscosity.
• Metabolism: Severe dyslipidemia, persistent metabolic disease, disruption of oxidative metabolism.
• Infectious and inflammatory illness: Necrotizing fasciitis, never ending inflammation, and low grade systemic inflammatory response syndrome.
• Sub-health and Anti-aging: Chronic fatigue treatment, amelioration of sleep quality, and elimination of long-term oxidation.
• Immune Dysfunction: Regulating prior immune response and improving low immunity.EBOO vs Traditional Ozone Therapy
  Once compared to the classic autohemotherapy and local ozone insufflation, EBOO has unique technical and therapeutic advantages:
• Total Blood Volume Treatment: It accomplishes total blood circulation therapy during one session that is more comprehensive than conventional methods of blood treatment involving only limited blood volume.
• Better Safety: Sealed sterile circuit prevents air pollution and transfers; accurate control of ozone concentration prevents excessive oxidation.
• Better Stability: Continuous countercurrent exchange allows for stable and uniform blood oxygenation and ozonation as well as stable treatment outcomes.
• Double Purification Effect: It combines oxidizing therapy and physical filtration.

6. Safety, contraindications and medical consensus
   According to the outcomes of more than 1200 cases of EBOO worldwide, the EBOO application is associated with low incidence of adverse events; there is only mild occasional vertigo that disappears in the short period of time.
   Absolute Contraindications: Serious anemia, coagulation problems, acute bleeding disease, severe organ failure, ozone intolerance, pregnancy.
   Relative Contraindications: Acute infection fever, instability of vital signs, and very high blood pressure.
   The international organizations such as ISCO3 (International Scientific Committee of Ozone Therapy) have recognized EBOO as an important and effective supportive procedure in medicine.
7. Conclusion
   Extracorporeal Blood Oxygenation and Ozonation (EBOO) is a new step forward in the development of ozone blood therapy due to the implementation of modern principles of extracorporeal circulation, hyperbaraxia, and ozone regulation; because of the application of EBOO blood purification treatment is performed, antioxidant activation, immune response modulation, and metabolism increasing are achieved. Due to high safety, standardization, and great clinical versatility, EBOO remains one of the promising researches and applications in modern alternative medicine.